## 1 CE 321 PRINCIPLES ENVIRONMENTAL ENGINEERING LAB WORKSHEET No. 1 Due: One (1) Week After Each Lab Section, respectively MICROBIOLOGY Environmental engineers employ microbiology in a variety of applications. Testing for coliform bacteria is used to assess whether pathogens may be present in a water or wastewater sample. Coliforms are a type of bacteria that live in the intestines of warm blooded mammals, such as humans and cattle. They are not pathogens, but if they are present in a sample, it is taken as an indication that fecal material from humans or cattle has contacted the water. If fecal material is present, pathogens may be present, too. In water treatment, coliform counts must average less than one colony per 100 milliliters of sample tested. In wastewater treatment, typical acceptable levels might be 200-colonies/100 mL. There are two standard ways to test for coliforms, the Most Probable Number test, MPN (also called the multiple tube fermentation technique, MTF) and the membrane filter test, MF. Several companies market testing systems that are somewhat simpler, but these cannot be used by treatment plants until they receive EPA approval. Two recently accepted methods are the Minimal Media Test (Colilert system), and the Presence-Absence coliform test (P-A test). Wastewater treatment plant operators study the microorganism composition of the activated sludge units in order to assess and predict the performance of the biological floc. A sample of mixed liquor from the aeration basin is examined under the microscope, and based on the relative predominance of a variety of organisms that might be present; the operator can tell if the BOD application rates and wasting rates are as they should be. For your worksheet, please submit the items requested below (10 pts. each): 1. Examine a sample of activated sludge under the microscope (To be done together in class). Use the Atlas, Standard Methods, or other references to identify at least 5 different organisms you observed. List them and sketch them neatly on unlined paper. Describe their motility and any other distinctive characteristics as you observed it. 2. Explain what types of organisms you might expect to find in sludge with a high mean cell residence time (MCRT), and explain why these would predominate over the other types. 3. How can the predominance of a certain kind of microorganism in activated sludge affect the settling characteristics of the sludge? Give several examples. 2 4. Explain why coliforms are used as “indicator organisms” for water and wastewater testing. Name two pathogenic bacteria, two pathogenic viruses, and one pathogenic protozoan sometimes found in water supplies. 5. There is also a test for fecal coliforms. Use your class notes and outside references to explain the distinctions between the tests for total and fecal coliforms. Explain why one would use the fecal coliform test instead of the test for total coliforms. 6. Using outside references, indicate typical coliform limits for surface waters used for swimming and fishing; potable water; and wastewater treatment plant effluent. 7). In the recent past, EPA instituted regulations designed to insure that Giardia are removed from the water. Using your text or other references, explain what kind of organism this is, and explain the way in which EPA has set standards to insure they are removed during water treatment. 8. What is meant by “population dynamics”? What two factors usually control the population dynamics of a mixed culture? 9. Use the MPN test data from the samples prepared for class prior to determine the number of coliforms present in the wastewater samples. Please show your work and explain your reasoning. Total Coliforms Raw Intermediate Effluent Sample Volume No. Positive No. Positive No. Positive 10 5 5 4 1 5 5 2 0.1 5 3 1 0.01 5 1 0 0.001 2 1 —- 0.0001 1 —- —- FecalColiforms Raw Intermediate Effluent Sample Volume No. Positive No. Positive No. Positive 10 5 5 2 1 5 4 0 0.1 5 2 1 0.01 1 0 0 0.001 0 0 —– 0.0001 2 —– —– 3 10. Use the membrane filter test data given in class to determine the number of total coliforms and fecal coliforms present in the sample. Please show your work and explain your reasoning. Total Coliforms Fecal Coliforms Dilution Colonies Dilution Colonies Raw Influent 0.1 mL/100 mL 58 1 mL/100 mL 47 Intermediate 1 mL/100 mL 13 10 mL/100 mL 28 Wetland Effluent 10 mL/100 mL 10 100 mL/100 mL 15

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## What research methods did they employ? Would all of these methods be acceptable today? Consider standards set by the Institutional Review Board (IRB).

Kinsey’s investigation went ahead of hypothesis and discussion to include … Read More...

## The following equation can be used to compute values of y as a function of x: y = b e?ax sin(b x) (0:012 x4 ? 0:15 x3 + 0:075 x2 + 2:5 x) where a and b are parameters. Write the equation for implementation with MATLAB, where a = 2, b = 5, and x is a vector holding values from 0 to =24 in increments of x = =40. Employ the minimum number of periods (i.e., dot notation) so that your formulation yields a vector for y. In addition, compute the vector z = y2 where each element holds the square of each element of y. Combine x, y, and z into a matrix w, where each column holds one of the variables, and display w using the short g format. In addition, generate a labeled plot of y and z versus x. Include a legend on the plot (use help to understand how to do this). For y, use a 1:5-point, dashdotted red line with 14-point, red-edged white-faced pentagram-shaped markers. For z, use a standard-sized (i.e., default) solid blue line with standard-sized, blue-edged, green-faced square markers.

For any additional help, please contact: info@checkyourstudy.com Call and Whatsapp … Read More...

## Overview The human body can regulate its function responding to the change of its environment. Temperature is one of the factors which can modulate the body function. Refer to the related lectures and other resources; answer the followed questions (question 1-5 need at least 400 words together): Q1 In case of cold weather how does human body detect the coldness? Explain the signal detection, delivery, processing and involved cells, tissues and organs.

The chief brain mechanisms for heat regulation are established … Read More...

## 5. What approaches can be taken to develop a supply chain infrastructure that provide and accurate view of overall channel performance?

Join forces in areas where we have a solid … Read More...

## Q3a. Describe into details two principles of access control.

ITU-T suggested standard X.800 defines access control as follows: “The … Read More...

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## Course: PHYS 5426 — Quantum Statistical Physics Assignment #1 Instructor: Gennady Y. Chitov Date Assigned: January 15, 2014 Due Date: January 29, 2014 Problem 1. Prove [a; f(a†)] = @f(a†) @a† (1) [a†; f(a)] = −@f(a) @a (2) for arbitrary function f of operator which admits a series expansion. The Bose creation/ annihilation operators satisfy the standard commutation relations [a; a†] ≡ aa† − a†a = 1 (3) Hint: From Eqs.(1,2) one can figure out the corresponding commutation relations for the powers of creation/annihilation operators and then prove them by the method of mathematical induction. Note that for an arbitrary operator Aˆ: @A^n @A^ = nAˆn−1. Problem 2. In the presence of a constant external force acting on a one-dimensional oscillating particle its Hamiltonian becomes that of the so-called displaced oscillator, and the Schr¨odinger equation ˆH (q) = E (q) of the problem (cf. lecture notes) can be written in terms of dimensionless variables as ( − 1 2 d2 d2 + 1 2 2 − √ 2 ) () = ” () ; (4) where q = √ ~ m! and E = ~!”. a). Write the Schr¨odinger equation (4) in terms of the creation/annihilation operators of the harmonic oscillator ( = 0) = √1 2 (a + a†) (5) d d = √1 2 (a − a†) (6) 1 Via a linear transformation to the new creation/annihilation operators ˜a†; ˜a preserving the bosonic commutation relations for ˜a†; ˜a map the problem (4) of the displaced oscillator onto that of a simple harmonic oscillator with new operators (˜a†; ˜a). b). Find the spectrum (eigenvalues) ” (E) of the displaced oscillator. c). Write the normalized eigenstates |n⟩ of the displaced Hamiltonian (4) via a† and the vacuum state |Θ◦⟩ of the new operators, i.e. ˜a|Θ◦⟩ = 0 (7) d). As follows from the completeness of the oscillator’s eigenstates, the vacuum state of the displaced oscillator |Θ◦⟩ can be related to the simple oscillator’s vacuum |0⟩ (i.e., a|0⟩ = 0) as |Θ◦⟩ = Ω(a†)|0⟩ (8) Find (up to a normalization factor) the operator function Ω(a†) relating two vacua. Hint: in working out Eqs.(7,8), employ Eqs.(1,2). Problem 3. Prove from the standard commutation relations ([ai; a † j ]∓ = ij , etc) that ⟨0|aiaja † ka † l |0⟩ = jkil ± ikjl (9) the sign depending on the statistics. Also calculate the vacuum expectation value ⟨0|ahaiaja † ka † l a† m |0⟩. Problem 4. In the formalism of second quantization the two-particle interaction term of the Hamiltonian for spinless fermions is given by ˆ V = 1 2 ∫ ∫ dxdy ˆ †(x) ˆ †(y)V(x; y) ˆ (y) ˆ (x) (10) For the short-ranged interaction V(x; y) = V(|x−y|) ≡ V(r) = e2 exp(−r)=r find ˆ V in the momentum representation. The field operators and the creation/annihilation operators in the momentum representation are related in the usual way, i.e., ˆ †(x) = ∫ dp (2)3 a†(p)e−ipx (11) Note that the limit → 0 recovers the Coulomb (long-ranged) interaction V(r) = e2=r. What is the Fourier transform V(q) of the Coulomb interaction? 2 Problem 5. The matrix elements of a two-particle interaction from the previous problem can be written as ⟨k3k4|V|k1k2⟩ = (2)3(k1 + k2 − k3 − k4)V(q) (12) where q ≡ k3−k1 is the momentum transfer. Show that the diagonal part of the interaction operator ˆ V found on the previous problem in the k-representation, arises from momentum transfers q = 0 and q = k2−k1. Write down the two interaction terms and identify them as direct (q = 0) and exchange (q = k2 − k1) interactions. Draw the corresponding Feynman diagrams. Problem 6. Find the first correction to the temperature dependence of the chemical potential of the degenerate ideal electron gas, assuming constant particle concentration ⟨N⟩=V . Express the result in terms of T and the zero-temperature chemical potential ◦. For the calculations the following formula (we set kB = 1) can be used: I ≡ ∫ ∞ 0 f(“)d” e(“−)=T + 1 = ∫ 0 f(“)d” + 2 6 T2f′() + O(T4) (13) 3

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